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Submitted: 10 Dec 2020
Accepted: 18 Dec 2020
ePublished: 30 Dec 2020
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Avicenna J Dent Res. 2020;12(4): 120-125.
doi: 10.34172/ajdr.2020.24
  Abstract View: 1090
  PDF Download: 599

Original Article

Effects of Low-Level Diode Laser on the Level of Interleukin 1-Beta in the Gingival Crevicular Fluid and the Incidence of Gingivitis Caused by the Use of Orthodontic Separators: A Randomized Clinical Trial

Sepideh Soheilifar 1 ORCID logo, Sara Soheilifar 2, Shayesteh Hatami 3, Farshid Vahdatinia 4, Vahid Molabashi 5, Mehrdad Hajiloui 6, Pouria Teymouri 7* ORCID logo

1 Assistant Professor, Department of Orthodontics, Implant Research Center, Hamedan University of Medical Sciences, Hamadan, Iran.
2 Periodontist, Private Office.
3 General dentist, Kermanshah, Iran.
4 General Dentist, Dental Implants Research Center, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
5 Associate Professor of Orthodontic Department, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
6 Department of Immunology, School of Medicine, Hamedan University of Medical Science, Hamedan, Iran.
7 General Dentist, Resident of Oral and Maxillofacial Surgery, Hamadan University of Medical Sciences, Hamadan, Iran.
*Corresponding Author: Correspondence to Pouria Teymouri, Phone: +98-939-114-6753, Email: , Email: pouria6753@gmail.com

Abstract

Background: In recent years, there has been an increased tendency for using dental lasers for the treatment of soft tissue problems. The aim of this study was to evaluate the effect of low-level diode laser (980 nm) on the level of interleukin 1 beta (IL-1β) in the gingival crevicular fluid (GCF) and the incidence of initial gingivitis caused by the use of orthodontic separators.

Methods: In this randomized clinical trial, 30 patients, who were beginning a fixed orthodontic treatment without gingivitis, were randomly assigned to control and diode laser radiation (980 nm wavelength, 3 J of energy, a density of 3 J/cm2 , a power of 0.2 W, and at a distance of 1 cm away from the tissue for 15 seconds on the buccal and palatal sides of the tooth) groups. The gingival index (GI) and bleeding on probing (BOP) were measured at the beginning of the study and one week after the treatment. The level of IL-1β was evaluated using an enzyme-linked immunosorbent assay at the beginning of the study and one week after the placement of the separator. Finally, the inter-group and intra-group statistical analyses were performed using independent and paired t tests, and P<0.05 was considered as the significance level.

Results: The evaluation of clinical variables in the entire mouth showed a slight clinical improvement in the experimental group although there was no significant difference between the two groups. No significant difference was observed between intra-group and inter-group evaluations of clinical indices in the studied specific teeth. Eventually, no difference was found between the two groups in terms of IL-1β changes.

Conclusions: In general, the single-diode laser radiation session is not effective in the treatment of gingivitis in patients undergoing orthodontic treatment. Thus, it is recommended to perform frequent laser radiation sessions in further studies.


Citation: Soheilifar S, Soheilifar S, Vahdatinia F, Hatami S Molabashi V, Hajiloui M, Teymouri P. Effects of low-level diode laser on the level of interleukin 1-beta in the gingival crevicular fluid and the incidence of gingivitis caused by the use of orthodontic separators: a randomized clinical trial. Avicenna J Dent Res. 2020;12(4):120-125. doi: 10.34172/ ajdr.2020.24.
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