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Submitted: 23 Jul 2018
Accepted: 03 Sep 2018
ePublished: 12 Sep 2018
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Avicenna J Dent Res. 2018;10(3): 101-106.
doi: 10.34172/ajdr.2018.21
  Abstract View: 955
  PDF Download: 716

Original Article

Effect of Trypsin/EDTA Conditioning on Marginal Microleakage of Class V Composite Restorations Bonded With a One-Step Selfetch Adhesive

Shahin Kasraei 1 ORCID logo, Matin Kordestani 2* ORCID logo, Ebrahim Yarmohammadi 3, Hesamaldin Pashaei 4

1 Professor of Department of Restorative Dentistry, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
2 Post Graduate Student, Department of Restorative Dentistry, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
3 Assistant professor, Department of Restorative Dentistry, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
4 Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
*Corresponding Author: *Correspondence to Matin Kordestani, DDS, Post Graduate Student, Department of Restorative Dentistry, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran. Tel: +98 9123481767, Fax: +98-81-38381085, Email: , Email: mkordestani3@gmail. com

Abstract

Background: Sodium hypochlorite and EDTA solutions are used in the preparation of dentin surfaces, which may increase the bond strength of the composite restorations and decrease their microleakage scores. This in-vitro experimental study aimed to assess the effect of dentin surface conditioning with EDTA/trypsin solution prior to bonding procedure with Clearfil S3 Bond on the microleakage of class V composite restorations.

Methods: Class V cavities were prepared on the buccal and lingual surfaces of 18 human premolars (n=18) and randomly assigned into two groups. In the control group, Clearfil S3 Bond was applied on the dentin surfaces. While in the experimental group, before applying Clearfil S3 Bond, the surfaces were conditioned with EDTA/trypsin solution. The cavities on the teeth were then incrementally filled with Filtek Z250 light-cure composite. Afterward, all the samples were thermocycled for 500 cycles. Next, the specimens were immersed in methylene blue solution for 72 hours. The restorations were sectioned buccolingually through the center of the restorations with a diamond disk. Then, microleakage at the tooth- restoration interface was evaluated in the enamel and dentin margins blindly using dye penetration under a stereomicroscope at ×40 magnification. Finally, data were statistically analyzed by Mann-Whitney U test.

Results: The results showed no significant differences between two groups regarding the microleakage scores of the enamel and dentin edges. In addition, in all sections and edges of the experimental group, decreased microleakage scores were attained compared to the control specimens (P<0.03).

Conclusions: Regarding the limitations of the present study, the preparation of dentin surfaces with EDTA/trypsin solution before applying Clearfil S3 Bond resulted in the decreased microleakage scores compared to unprepared surfaces.


Citation: Kasraei S, Kordestani M, Yarmohammadi E, Pashaei H. Effect of trypsin/EDTA conditioning on marginal microleakage of class V composite restorations bonded with a one-step self-etch adhesive. Avicenna J Dent Res. 2018;10(3):101-106. doi: 10.15171/ajdr.2018.21. 
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