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Submitted: 04 Dec 2024
Revision: 16 Mar 2025
Accepted: 16 Mar 2025
ePublished: 29 Sep 2025
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Avicenna J Dent Res. 2025;17(3): 154-159.
doi: 10.34172/ajdr.2187
  Abstract View: 45
  PDF Download: 22

Original Article

Comparative In Vitro Study of Antimicrobial Efficacy of Sodium Hypochlorite, Normal Saline, and Hekla Lava Tooth Powder as Root Canal Irrigants Against Enterococcus faecalis

Banu Jothi A 1 ORCID logo, Rajmohan M 2* ORCID logo, Savithri S 3, Prabu D 4, Lubna Fathima 5, Sindhu R 5, Dinesh Dhamodhar 2, Indira Nehru 5, Hari Priya R 1

1 Postgraduate, Public Health Dentistry, SRM Dental College, Bharathi Salai,Chennai, India
2 Master of Dental Surgery, Reader, Department of Public Health Dentistry, SRM Dental College, Rampuram, Chennai, India
3 Professor and Head, Department of Microbiology, SRM Dental College, Bharathi Salai, Chennai, India
4 Master of Dental Surgery, Professor and Head, Department of Public Health Dentistry, SRM Dental College, Bharathi Salai, Chennai, India
5 Master of Dental Surgery, Senior Lecturer, Department of Public Health Dentistry, SRM Dental College, Bharathi Salai, Chennai, India
*Corresponding Author: Rajmohan M, Email: researchphdsrm@gmail.com

Abstract

Background: Enterococcus faecalis is a bacterium commonly associated with persistent endodontic infections. Hekla Lava has healing properties that enhance its antibacterial effects, making it a potential alternative to traditional irrigants, such as sodium hypochlorite (NaOCl). E. faecalis is particularly resilient and can form biofilms, thus complicating treatment outcomes; therefore, exploring effective irrigants is crucial for improving root canal disinfection strategies. This research aimed to assess whether Hekla Lava tooth powder (HLTP) can provide sufficient antimicrobial efficacy to support successful endodontic treatments. It evaluated the antibacterial efficacy of NaOCl, normal saline (NS), and HLTP as a root canal irrigant against E. faecalis.

Methods: The in vitro study was conducted in an extracted non-carious human tooth. The treatment groups were treated with NaOCL (group A), NS (group B), and HLTP (group C) for the irrigation of the root canal for 5 minutes and 48 hours.

Results: The results of the study demonstrated significant differences in the antimicrobial efficacy of the three treatment groups at both time points. HLTP showed a decrease in colony-forming units from a mean initial count of 59.35±5.80 after 5 minutes to 40.47±3.81 after 48 hours, indicating that its use was better when compared to NS.

Conclusion: The findings reaffirm that the use of HLTP as an irrigant is preferable compared to NS due to its superior antimicrobial properties against E. faecalis. NS lacks substantial antimicrobial effects, making it less effective for disinfection in root canal procedures. This study highlights the potential of HLTP as a more effective alternative for improving treatment outcomes in endodontics, suggesting a shift toward herbal and natural solutions in dental care practices.



Please cite this article as follows: Banu Jothi A, Rajmohan M, Savithri S, Prabu D, Lubna Fathima, Sindhu R, et al. Comparative in vitro study of antimicrobial efficacy of sodium hypochlorite, normal saline, and Hekla Lava tooth powder as root canal irrigants against Enterococcus faecalis. Avicenna J Dent Res. 2025;17(3):154-159. doi:10.34172/ajdr.2187
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