Abstract
Background: Estrogen as a steroid hormone plays a role in the differentiation and growth of various tissues, such as the breast, endometrium,
colon, and prostate, as well as their cancers. The literature offers different and partly contradictory findings concerning
the investigations of estrogen receptor (ER) expression in salivary gland tumors.
Objectives: The aim of this study was to investigate the expression of ER in two main types of malignant salivary gland tumors,
namely adenoid cystic carcinoma (ACC) and mucoepidermoid carcinoma (MEC), in an Iranian population using immunohistochemical
techniques.
Patients and Methods: After reviewing the patients’ records in the departments of pathology in some hospitals in the city of Yazd
covering all native and southern-region patients, out of all malignant salivary glands tumors, 40 specimens were retrieved. These
included 24 ACCs and 16 MECs. Following the preparation of 4 µm sections and the deparaffinization and dehydration steps, the
immunohistochemical process was conducted using the antibody against estrogen receptor α (Clone1D5, DAKO, Denmark). The
prepared slides were then observed under a light microscope, and the stained nuclei were counted. The data were analyzed using
SPSS 17 and the generalized Fisher exact test.
Results: For ACC, only one specimen expressed the ER, and this was assessed as moderate. No specimens of MEC expressed the ER.
The comparison of ER expression between the two groups was not meaningful (P = 0.99).
Conclusions: Regarding the findings of this study, it can be concluded that ERα possibly cannot play an important role in the
tumorigenesis and prognosis of the investigated malignant salivary gland tumors.