Hamadan University of Medical Sciences Avicenna Journal of Dental Research 2423-7582 6 1 2014 06 25 The Effect of Chlorhexidine on Push-out Bond Strength of Mineral Trioxide Aggregate 21 24 10.17795/ajdr-21195 EN Alireza Adl Nooshin Sadat Shojaee Fereshte Sobhnamayan Neda Sadat Shojaee Journal Article 10.17795/ajdr-21195 2014 06 02 2014 06 15 Background: Trioxide Aggregate (MTA) has been widely used in root canal therapy. MTA has been mixed with chlorhexidine to increase its antimicrobial effect. Objectives: The aim of this study was to evaluate the effect of chlorhexidine (2%) on push-out bond strength of Mineral Trioxide Aggregate (MTA). Materials and Methods: Sixty dentin disks with a thickness of 1.5 ± 0.2 mm and lumen size of 1.3 mm were prepared. Dentin disks were randomly divided into four groups (n = 15), and their lumens were filled with MTA mixed with distilled water (groups 1 and 3) or with chlorhexidine 2% (groups 2 and 4). Specimens were incubated at 37°C for 3 days (groups 1 and 2) or 21 days (groups 3 and 4). Bond strengths of the MTA-treated dentin surfaces were evaluated using a universal testing machine, and bond failure on the disks was examined by light microscope. Data was analyzed using Kruskal-Wallis H test (P = 0.976). Results: There were no statistically significant differences between all the experimental groups. The mode of bond failure was predominantly mixed for distilled water groups and cohesive for CHX groups. Conclusions: This study suggested that chlorhexidine had no negative effect on the bond strengths of MTA-treated dentin.