Hamadan University of Medical Sciences Avicenna Journal of Dental Research 2423-7582 15 3 2023 09 30 The Effect of Different Percentages of Nano-bioactive Glass in the Synthesized CPP/ACP Paste on the Remineralization of Demineralized Enamel 92 96 10.34172/ajdr.527 EN Zahra Khamverdi https://orcid.org/0000-0001-9623-5573 Abbas Farmani Maryam Farhadian Alireza Mazaheri https://orcid.org/0000-0002-1654-4155 Journal Article 10.34172/ajdr.527 2021 10 12 2021 10 28 Background: The preventive treatments of primary caries lesions are essential for preventing destructive damage to the tooth structure. One of the common treatments is the application of casein phosphopeptide-amorphous calcium phosphate (CCP/ACP) paste on the enamel surface. The aim of this study was to investigate the effect of different percentages of nano-bioactive glass (nBG) incorporation into synthesized CPP/ACP paste on the remineralization of demineralized enamel. Methods: In general, 24 extracted human intact premolar teeth were selected, and their crowns were removed for this purpose. Each crown was cut into two halves, and each half was considered as a sample. The samples were placed in a demineralizing solution at a pH rate of 4.6 for 8 hours, in artificial saliva for 1 hour, and again in a remineralizing solution at a pH rate of 7 for 15 hours. The pH cycling was performed for 14 days to demineralize the enamel surface. The samples were randomly divided into 3 groups (n=16), including G1 (without treatment), G2 (treated with synthesized CPP/ ACP paste containing 5% nanobioglass), and G3 (treated with synthesized CPP/ACP paste containing 10% nanobioglass). The paste was then placed directly on the surface of the demineralized enamel for 4 minutes (twice a day for 28 days). The samples were subjected to the Vickers microhardness test. Finally, data were analyzed using SPSS (version 19) and the analysis of variance and Tukey’s tests (α = 0.05). Results: There was a significant difference between microhardness values in G1 and G2, as well as G1 and G3 (P<0.05). However, no statistically significant difference was observed between G2 and G3 (P>0.05). Conclusions: The results showed adding bioactive glass into synthetic CPP/ACP paste increases enamel remineralization in spite of the percentage of bioactive glass incorporation.