Hamadan University of Medical Sciences Avicenna Journal of Dental Research 2423-7582 12 2 2020 06 29 The Effects of the Grape Seed Extract on Streptococcus mutans and Candida albicans: An In Vitro Study 35 39 10.34172/ajdr.2020.08 EN Masoumeh Aslanimehr https://orcid.org/0000-0003-2591-8523 Ashraf Alizadeh Faezeh Azmoudeh https://orcid.org/0000-0003-2613-355X Journal Article 10.34172/ajdr.2020.08 2020 06 01 2020 06 18 Background: Oral pathogens can affect a large population and the general health of individuals. Based on the evidence, the grape seed extract (GSE) contains herbal compounds that have the ability tosuppress oral pathogens associated with caries and fungal diseases. In this regard, the current studyaimed at evaluating the effect of GSE on Streptococcus mutans and Candida albicans. Methods: The grape (Vitis vinifera L.) seed was used in this in-vitro experimental study. After thepreparation of methanolic GSE, its effect on S. mutans and C. albicans was assessed at 0.25-256 mg/mL concentrations. Then, the minimum inhibitory concentration (MIC) and minimum bactericidalconcentration (MBC) were determined by macro broth dilution methods. Finally, data were analyzedby SPSS, version 20. Results: The results showed that GSE inhibits the growth of S. mutans and C. albicans. In addition, theMIC and MBC of the extract against S. mutans and C. albicans were 2 and 8 mg/mL, as well as 32 and64 mg/mL, respectively. Conclusions: In general, GSE had a significant antimicrobial effect on S. mutans, while it only affected C. albicans at high concentrations.