Abstract
Background: Blood contamination could interfere with the bonding of resin materials to different substrates.
Objectives: The purpose of this study was to evaluate the effects of various methods of removing blood contamination between
composite resin increments on resin-resin micro-shear bond strength.
Methods: 90 composite blocks (Valux Plus, 3M ESPE) measuring 2 × 2 × 8 mm were prepared in 6 groups: one group was not
contaminated as the control (group 1); for other groups the surface was contaminated with blood then rinsed and dried. One group
only received this treatment (group 2) and others were etched with phosphoric acid (group 3), phosphoric acid and a bonding agent
(Margin Bond, Coltene) (group 4), cleaned with 98% ethanol (group 5), and had 0.5 mm of their outer surface removed (group 6).
Next, the second increments of composite were applied in the form of cylinders of 0.7 mm diameter and 1 mm height on the top
surfaces of the specimens and were then light cured. Specimens underwent micro-shear bond strength testing. Data were analyzed
by Kolmogorov-Smirnov and analysis of variance (ANOVA) tests.
Results: The mean value of bond strength was 23 ± 3.60 MPa for the control group, 17.89 ± 6.52 MPa for group 2, 19.40 ± 6.08 MPa
for group 3, 20.20 ± 5.72 MPa for group 4, 20.01 ± 6.83 MPa for group 5, and 19.10 ± 6.20 MPa for group 6. There were no significant
differences between the groups.
Conclusions: All the decontamination methods in this study could increase the resin-resin bond strength to the level of the control
group.